Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging

Abstract Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization the spatial-temporal evolution cell state in 3D culture subsequent 2D dissociated based on protein expression levels localizations is essential to understanding differentiation, disease phenotypes, sample-to-sample variability. Here, we apply PR obe-based I maging for S equential M ultiplexing (PRISM) facilitate multiplexed imaging with facile, rapid exchange probes analyze iPSC-derived cortical motor neuron that are psychiatric neurodegenerative models, using over ten targets. Our approach permits analysis composition, functional marker complex stem-cell derived cultures. Furthermore, our amenable automation, offering principle ability scale-up dozens targets samples.